th; min-height: 1em; text-align: justify; line-height: 1.75em; font-size: 14px; text-shadow: none; letter-spacing: 1.5px; font-family: 微软雅黑, "Microsoft YaHei"; box-sizing: border-box !important; overflow-wrap: break-word !important;">4. Claudin18.2 VLP samples were stained with anti-Claudin18.2 antibodyat 1ug/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.Claudin18.2卵白Figure 1. Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2μg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody (BME100075) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6ng/ml.Figure 2. Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGEClaudin18.2是当下最热点的靶点之一，针对于该靶点开发的立异药包孕单抗、双抗、CAR-T、ADC四种。Claudin18.2是四次跨膜卵白，属在Claudins卵白家族，该家族是一类存于在上皮以及内皮慎密毗连中的整合素膜卵白，是组成慎密毗连最要害的卵白之一，可以或许有用维持上皮细胞以及内皮细胞的极性。Claudins卵白家族的重要功效是调治樊篱布局的渗入性。Claudin18.2是一种胃特异性的慎密毗连卵白，于很多癌症中都有表达，尤为高表达在消化体系恶性肿瘤，包孕胃癌，胰腺癌，食管癌等。于肿瘤中，Claudin18.2介入肿瘤细胞的增殖分解以及迁徙。Claudin18.2只于癌细胞中广泛表达使患上它成为一个抱负的肿瘤免疫靶点。01缔码全长Claudin18.2卵白针对于Claudin18.2，缔码生物哄骗公司的全长多跨膜卵白表达平台：VLP以及Synthetic Nanodisc，基在哺乳动物表达体系，乐成表达功效性Claudin18.2全长多跨膜卵白，并颠末ELISA或者流式阐发验证，注解咱们的Claudin18.2全长卵白产物具备自然活性以及构象，可以与响应单克隆抗体特异性联合。02运用场景抗体筛选Claudin18.2全长卵白具备优秀的免疫原性，可筛选出更多表位的抗体功效检测Claudin18.2全长卵白可用在抗体或者配体亲以及力检测CAR-T阳性率检测Claudin18.2全长卵白可与该靶标的CAR-T细胞外貌的scFv联合，检测CAR-T阳性率03产物列表靶点货号产物描写运用Claudin18.2FLP100014Human Claudin18.2 full length protein-synthetic nanodiscELISA,Flow CytFLP100006Human Full length Claudin 18.2 Protein-VLPELISA,Flow CytFLP100017Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)ELISA,Flow Cyt04验证数据Claudin18.2-EGFP卵白Figure 1. ELISA plates were pre-coated with 0.5μg/per well purified human CLDN18.2-EGFP-VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (BME100075) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-EGFP-VLP is 877.5ng/ml.Figure 2. FACS analysis of CLDN18.2 VLPA. Negative Control 1: Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by streptavidin-APC antibody.D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).Claudin18.2卵白Figure 1. Elisa data: ELISA plates were pre-coated with 0.5ug/per well purified human Claudin 18.2 VLP. Serial diluted Anti-Claudin18.2 monoclonal antibody (Zolbetuximab biosililar; IMAB362) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for IMAB362 binding with Claudin18.2 is 15.37ng/ml.Figure 2. Flow virometry analysis of Claudin18.2-VLP particles1. Negative Control 1: Claudin18.2 VLP samples were stained only with Goat anti-human IgG Fc-PE secondary antibody.2. Negative Control 2: Control VLP samples were stained with anti-Claudin18.2 antibodyat 1ug/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.3. Negative Control 3: Claudin18.2 VLP samples were stained with anti-BCMA antibodyat 1ug/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.